Adipogenic Differentiation of GPCs

The ability of GPCs to differentiate into multiple stromal lineages was tested as previously described (Mohamed-Ahmed et al., 2018 (link)). Briefly, for adipogenic differentiation, cells in HPL and FBS were cultured in StemPro^® adipogenic differentiation medium (Invitrogen) or standard growth medium (control). After 21 days, cells were fixed with 4% paraformaldehyde (PFA) for 10 min at RT and intracellular lipid formation was assessed via Oil red O staining (Sigma-Aldrich, St. Louis, MO, United States).

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Shanbhag S., Suliman S., Bolstad A.I., Stavropoulos A, & Mustafa K. (2020). Xeno-Free Spheroids of Human Gingiva-Derived Progenitor Cells for Bone Tissue Engineering. Frontiers in Bioengineering and Biotechnology, 8, 968.

Publication 2020

StemPro® adipogenic differentiation medium Oil red O staining

Adipogenic Cells Gpcs Growth medium Intracellular Lipid formation Paraformaldehyde

Corresponding Organization : University of Bergen

Other organizations : Malmö University, University of Geneva

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Variable analysis

independent variables

Cell culture conditions: HPL (human platelet lysate) and FBS (fetal bovine serum)

dependent variables

Adipogenic differentiation (lipid formation) assessed via Oil red O staining

control variables

Standard growth medium (control condition)

controls

Negative control: Cells cultured in standard growth medium

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