MALDI-TOF Mass Spectrometry of Bed Bug Profiles

The spectral profiles obtained from the bed bugs’ heads (adults) and cephalothoraxes (immature stage) were obtained using a Microflex LT MALDITOF mass spectrometer with FlexControl software (version 3.3, Bruker Daltonics). The spectra were acquired in a positive linear mode at a laser frequency of 50 Hz. The accelerating voltage was 20 kV, and the extraction delay time was 200 ns. Each spectrum corresponded to the ions obtained from the 240 laser shots performed in six regions at the same location and acquired automatically using the AutoXecute Flex Control software (version 2.4; Bruker Daltonics). The MS profiles were visualised using the FlexAnalysis v3.4 software, MALDI Biotyper Compass Explorer v4.1.70 (Bruker Daltonics), and ClinProTools v3.0 software (Bruker Daltonics) was used for data processing^{49 (link)}.

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Hasnaoui B., Bérenger J.M., Delaunay P., Diarra A.Z., Ndiaye E.H., M’madi S.A., Masotti N., Sevestre J, & Parola P. (2023). Survey of bed bug infestations in homeless shelters in southern France. Scientific Reports, 13, 12557.

Publication 2023

FlexControl software AutoXecute Flex Control software FlexAnalysis v3.4 software MALDI Biotyper Compass Explorer v4.1.70 ClinProTools v3.0 software

Adults Bed bugs Heads Ions Maldi

Corresponding Organization :

Other organizations : Institut de Recherche pour le Développement, Aix-Marseille Université

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Variable analysis

independent variables

Spectral profiles obtained from bed bugs' heads (adults) and cephalothoraxes (immature stage)

dependent variables

Ions obtained from the 240 laser shots performed in six regions at the same location

control variables

Positive linear mode at a laser frequency of 50 Hz
Accelerating voltage of 20 kV
Extraction delay time of 200 ns

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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