Microscopy Chamber Preparation for Tubulin Immobilization
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Corresponding Organization :
Other organizations : Université Paris Sciences et Lettres, Université Paris-Saclay, Centre National de la Recherche Scientifique, Institut Curie, Czech Academy of Sciences, Institute of Biotechnology
Variable analysis
- Silanisation of glass coverslips
- Incubation of flow channels with anti-β-tubulin (TUBB) antibody
- Blocking of glass surface with Pluronic-F127
- Immobilization of microtubules at the glass surface
- Size of glass coverslips (18 and 24 mm, Marienfield High Precision)
- Dimensions of flow channels (~2.5 mm wide parallel flow channels)
- Concentration of anti-β-tubulin (TUBB) antibody (30 μg/ml)
- Concentration of Pluronic-F127 (1% in PBS)
- Duration of incubation with anti-β-tubulin (TUBB) antibody (5 min)
- Duration of blocking with Pluronic-F127 (at least 30 min)
- Temperature (room temperature)
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