The liver tissues of mice were fixed in 10% formalin, paraffinized, sliced and subjected to haematoxylin and eosin (H&E) staining. The paraffin-embedded liver was frozen using a cryostat and cut into 4-µm sections, fixed in 4% formalin, and then stained with oil red O/60% isopropanol solution (Thermo Fisher Scientific, Waltham, MA), and counterstained with haematoxylin, followed by observation utilizing a Zeiss Axioplan 2 upright microscope (Zeiss) [28 (link)]. In terms of Masson staining, sections were stained with iron haematoxylin, eosin acid fuchsin dye solution, and counterstained with aniline blue dye solution, followed by observation under a Zeiss Axioplan 2 upright microscope [29 (link)].
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