Quantifying Cell Proliferation via BrdU Incorporation

Cells were cultured as described in [7 (link)]. For cell proliferation analysis, the BrdU incorporation protocol was executed as detailed [7 (link)]. The percentage of BrdU-positive cells was assessed using a FACSCanto II flow cytometer. The data were analyzed using FlowJo software (version 7.6.1). BrdU incorporation was also observed by immunofluorescence, as in [7 (link)]. The coverslips were incubated with the primary antibody BrdU (Bu20a; diluted 1:200; #5292; Cell Signaling Technology) overnight at 4 °C. The goat anti-mouse Alexa Fluor 594 (diluted 1:150; A11032, Thermo Fisher Scientific) was used as the secondary antibody.

Free full text: Click here

Pádua D., Figueira P., Pinto M., Maia A.F., Peixoto J., Lima R.T., Pombinho A., Pereira C.F., Almeida R, & Mesquita P. (2023). High-Throughput Drug Screening Revealed That Ciclopirox Olamine Can Engender Gastric Cancer Stem-like Cells. Cancers, 15(17), 4406.

Publication 2023

BrdU (Bu20a; Alexa Fluor 594

Alexa 594 Antibody Brdu Cell proliferation Goat Immunofluorescence Mouse

Corresponding Organization : Universidade do Porto

Other organizations : Lund University

Top 5 similar protocols

Variable analysis

independent variables

Cell culture conditions as described in [7 (link)]

dependent variables

Percentage of BrdU-positive cells
BrdU incorporation observed by immunofluorescence

control variables

Cell proliferation analysis protocol as detailed in [7 (link)]
Primary antibody BrdU (Bu20a; diluted 1:200; #5292; Cell Signaling Technology) used for immunofluorescence
Goat anti-mouse Alexa Fluor 594 (diluted 1:150; A11032, Thermo Fisher Scientific) used as the secondary antibody for immunofluorescence

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!