Wound healing migration assays were performed as previously described [21 (link)]. A total of 105 cells were seeded into six-well plates. At 80% confluency, the cell monolayer was wounded with a 200 μL pipette, washed with PBS and medium replaced. Images were taken at 0 h, 24 h, 48 h, and 72 h intervals. Images were taken using Motic Images Plus v.2.0 Software (Motic, Richmond, BC, Canada).
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