ATX and IL-6 protein levels were quantified with dedicated ELISA kits according to the manufacturer’s instructions (R&D Systems Inc., Minneapolis, MN, USA, and Invitrogen, ThermoFisher Scientific, Waltham, MA, USA, respectively). Measurements were performed in a blinded fashion in triplicate using a Triturus automated analyzer (Grifols, Barcelona, Spain). The presented results on ELISA quantification of soluble E-selectin (sE-sel) and P-selectin (sP-sel), ICAM, and ANG2 in the same samples, has been reported previously [33 (link)].
Nikitopoulou I., Fanidis D., Ntatsoulis K., Moulos P., Mpekoulis G., Evangelidou M., Vassiliou A.G., Dimakopoulou V., Jahaj E., Tsipilis S., Orfanos S.E., Dimopoulou I., Angelakis E., Akinosoglou K., Vassilaki N., Tzouvelekis A., Kotanidou A, & Aidinis V. (2021). Increased Autotaxin Levels in Severe COVID-19, Correlating with IL-6 Levels, Endothelial Dysfunction Biomarkers, and Impaired Functions of Dendritic Cells. International Journal of Molecular Sciences, 22(18), 10006.
Ang2 E selectin Elisa Icam Il 6 protein P selectin Triturus
Corresponding Organization : Alexander Fleming Biomedical Sciences Research Center
Other organizations :
Evangelismos Hospital, National and Kapodistrian University of Athens, Pasteur Hellenic Institute, General University Hospital of Patras
No positive or negative controls were explicitly mentioned.
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