ATX and IL-6 protein levels were quantified with dedicated ELISA kits according to the manufacturer’s instructions (R&D Systems Inc., Minneapolis, MN, USA, and Invitrogen, ThermoFisher Scientific, Waltham, MA, USA, respectively). Measurements were performed in a blinded fashion in triplicate using a Triturus automated analyzer (Grifols, Barcelona, Spain). The presented results on ELISA quantification of soluble E-selectin (sE-sel) and P-selectin (sP-sel), ICAM, and ANG2 in the same samples, has been reported previously [33 (link)].
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