Dominant Negative FoxO1 in Insulin-Secreting Cells
Corresponding Organization : Columbia University
Variable analysis
- Transduction of MIN6 cells with adenovirus encoding dominant negative FoxO1 and GFP
- Treatment of MIN6 cells with FoxO1 inhibitor (AS18420856, 100 nM)
- Gene expression analysis
- MIN6 cell culture medium (DMEM with 4.5 g/L glucose and L-glutamine without sodium pyruvate, containing 15% fetal bovine serum, penicillin-streptomycin, and 0.05 mM 2-mercaptoethanol)
- HEK293 cell culture medium (DMEM with 1.0 g/L glucose and L-glutamine without sodium pyruvate, containing 10% fetal bovine serum and penicillin-streptomycin)
- Islet culture medium (RPMI 1640 containing 10% fetal bovine serum with penicillin-streptomycin)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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