As reported (Tanaka et al, 2006 ; Zaky et al, 2008 (link); Watari et al, 2012 (link)), we examined five microsatellite loci on chromosomes for MSI based on the revised Bethesda panel (Umar et al, 2004 (link)) as follows: 2p (BAT26), 4q (BAT25), 2p (D2S123), 5q (D5S346) and 17p (D17S250). The polymerase chain reaction (PCR) products were evaluated for MSI by capillary electrophoresis using an ABI PRISM 310 Genetic analyser (Applied Biosystems, Foster City, CA, USA) and automatic sizing of the alleles using a GeneMapper (Applied Biosystems). The MSI status was judged according to previous reports (Supplementary Figure S2 ) (Tanaka et al, 2006 ; Zaky et al, 2008 (link); Watari et al, 2012 (link)).
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Microsatellite Instability Analysis Protocol
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Other organizations : Hyogo Medical University, Rutgers, The State University of New Jersey
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Variable analysis
independent variables
- Microsatellite loci on chromosomes (2p (BAT26), 4q (BAT25), 2p (D2S123), 5q (D5S346) and 17p (D17S250))
- Microsatellite instability (MSI) status
- Revised Bethesda panel (Umar et al., 2004)
- Capillary electrophoresis using an ABI PRISM 310 Genetic analyzer (Applied Biosystems, Foster City, CA, USA)
- Automatic sizing of the alleles using a GeneMapper (Applied Biosystems)
- Positive controls: Not explicitly mentioned
- Negative controls: Not explicitly mentioned
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