Protocol detail

Screening and Cultivation of Spore-Forming Bacillus

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Bacillus subtilis of TLRI 211-1 was screened from the activated sludge at TLRI. After species identification, high temperature resistance (50°C), and spore-producing ability test, the B. subtilis was selected and inoculated in Tryptic Soy Broth culture media and placed in a 30°C incubator for 24 hours. After a process of adjusting with food grade silicon dioxide, mixing well, centrifuging to remove the upper liquid and air drying in a 40°C oven, the total content of viable sporulated TLRI 211-1 bacteria used in this experiment was 1×10⁹ CFU/g. Commercial B. amyloliquefaciens (CML. B. amyloliquefaciens) used in the treatment 4 was provided by Yungstrong, Vetnostrum Animal Health Co., Ltd, (Hsinchu, Taiwan) and adjusted to the content of 1×10⁹ CFU/g.

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Tsai M.Y., Shih B.L., Liaw R.B., Chen W.T., Lee T.Y., Hung H.W., Hung K.H, & Lin Y.F. (2023). Effect of dietary supplementation of Bacillus subtilis TLRI 211-1 on laying performance, egg quality and blood characteristics of Leghorn layers. Animal Bioscience, 36(4), 609-618.

Publication 2023

Ability test Animal Bacillus subtilis Bacteria Culture media Food High temperature Silicon dioxide Sludge Spore Tryptic soy broth

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Variable analysis

independent variables

Bacillus subtilis of TLRI 211-1
Commercial B. amyloliquefaciens (CML. B. amyloliquefaciens)

dependent variables

High temperature resistance (50°C)
Spore-producing ability

control variables

Tryptic Soy Broth culture media
30°C incubator
Food grade silicon dioxide
40°C oven

controls

Positive control: Commercial B. amyloliquefaciens (CML. B. amyloliquefaciens) provided by Yungstrong, Vetnostrum Animal Health Co., Ltd, (Hsinchu, Taiwan)
Negative control: Not mentioned

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