Synthesis and Purification of 2-Oxo-3-pentynoate

All chemicals, biochemical, and enzyme substrates were obtained from sources described elsewhere.^{19 (link),20} 2-Oxo-3-pentynoate (2-OP) was synthesized as reported.^{21 (link)} Chromatographic resins (DEAE Sepharose fast flow and Bio-Gel P60 gel beads) and columns (PD-10 Sephadex G-25M, Econo-Column, and Spectra/Chrom LC) were acquired from sources described elsewhere.^{19 (link)} The Amicon stirred cell concentrators and the ultrafiltration membranes (10,000 Da, MW cutoff) were purchased from EMD Millipore Inc. (Billerica, MA). InstantBlue for staining SDS-PAGE gels was obtained from C.B.S. Scientific Company, Inc. (Del Mar, CA).

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Baas B.J., Medellin B.P., LeVieux J.A., de Ruijter M., Zhang Y.J., Brown S.D., Akiva E., Babbitt P.C, & Whitman C.P. (2019). Structural, Kinetic, and Mechanistic Analysis of an Asymmetric 4-Oxalocrotonate Tautomerase Trimer. Biochemistry, 58(22), 2617-2627.

Publication 2019

DEAE Sepharose fast flow PD-10 Sephadex G-25M Econo-Column Spectra/Chrom LC Amicon stirred cell concentrators ultrafiltration membranes (10,000 Da, MW cutoff)

Cell Chrom Chromatographic Deae Enzyme Gels Membranes Resins Sds page Sephadex g Sepharose Ultrafiltration

Corresponding Organization : The University of Texas at Austin

Other organizations : University of California, San Francisco

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Variable analysis

independent variables

Synthesis of 2-Oxo-3-pentynoate (2-OP)

dependent variables

Not explicitly mentioned

control variables

All chemicals, biochemical, and enzyme substrates were obtained from sources described elsewhere.
Chromatographic resins (DEAE Sepharose fast flow and Bio-Gel P60 gel beads) and columns (PD-10 Sephadex G-25M, Econo-Column, and Spectra/Chrom LC) were acquired from sources described elsewhere.
The Amicon stirred cell concentrators and the ultrafiltration membranes (10,000 Da, MW cutoff) were purchased from EMD Millipore Inc. (Billerica, MA).
InstantBlue for staining SDS-PAGE gels was obtained from C.B.S. Scientific Company, Inc. (Del Mar, CA)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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