Intestinal Permeability Evaluation

We recruited 10 healthy volunteers (5 females) who were given instructions to not consume any steroids (6 weeks prior to the testing), medications affecting gastrointestinal transit or permeability (7 days prior to the testing) and artificial sweeteners, lactulose, mannitol (2 days prior to the testing)and during the 24 hour testing period. After baseline urine collection, three saccharides {100 mg ¹³C mannitol, 100 mg ¹²C (regular) mannitol, and 1000 mg lactulose} dissolved in 250 ml of water were administered. Urine collections were pooled for 0-2, 2-8 and 8-24 hours following administration of test sugars and excreted sugar concentrations were measured by HPLC-tandem MS.