Glutamate Toxicity in Murine Neurons

Vector control and human SelH-transfected murine hippocampal neuronal HT22 cells (V-HT22 and SelH-HT22, respectively) were cultured in Dulbecco's Modified Eagle Medium (DMEM)/F12, containing 10% fetal bovine serum (FBS, HyClone Cell Culture and Bioprocessing), 100 nM streptomycin /penicillin (HyClone Cell Culture and Bioprocessing), and cultivated at 90% relative humidity in 5% CO₂ at 37 ^○C. The culture medium was renewed every 2 days. Glutamate toxicity was induced by incubating the V-HT22 and SelH-HT22 cells with 6 mM Glutamate (Sigma-Aldrich Co.) for 24 h. Cell viability was assessed in 96-well cell culture plates. The transfection procedures and efficacy of transfection have been previously reported 9 (link),10 (link).