Integrin-Expressing REF52 and HFF Cells

REF52 cells, stably transfected to express β3-integrin with EGFP tag on its intracellular domain (Dr. Bernhard Wehrle-Haller, University of Geneva, Switzerland) [45] (link), [48] (link)–[50] (link), were cultured in Dulbecco’s modified Eagle’s media (DMEM) (Gibco, Invitrogen) supplemented with 10% fetal calf serum (Gibco, Invitrogen) and 1% penicillin–streptomycin (Gibco, Invitrogen). HFF cells (PromoCell) were cultured in fibroblast growth medium (PromoCell). For experiments, the culture media for HFF cells were changed to the GlutaMAX-1 containing DMEM (Gibco, Invitrogen) supplemented with 10% newborn calf serum (Gibco, Invitrogen) and 1% penicillin–streptomycin (Gibco, Invitrogen). All cells were cultured in culture flask (BD Biosciences) in a humidified incubator at 37°C with 5% CO₂ and 95% air for several passages before experiments.