Conditional Tspan12 Knockout Mice

All animal work was done in accordance to the Association for Research in Vision and Ophthalmology statement for the Use of Animals in Ophthalmic and Vision Research and with approval from the Institutional Animal Care and Use Committee at the University of Colorado. Animals of both genders were used for all studies. The following Cre-driver lines were used: Tg(Cdh5-cre/ERT2)1Rha,^{50 (link)} Pdgfrb(BAC)−CreERT2,^{51 (link)} and Rx-Cre,^{52 (link)} the latter kindly provided by Drs Eric Swindell and Milan Jamrich. mT/mG reporter mice were obtained from Jackson Laboratories. Conditional knockout mice were generated by first breeding Tspan12^+/− mice with each Cre-driver line (heterozygous for the transgene), then breeding Tspan12^+/−; Cre⁺ mice with Tspan12^flox/flox mice. The alleles Tspan12^flox (Tspan12^tm1.1Hjug) and Tspan12⁻ (Tspan12^tm1.2Hjug) were generated by gene targeting as described below.

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Zhang C., Lai M.B., Pedler M.G., Johnson V., Adams R.H., Petrash J.M., Chen Z, & Junge H.J. (2018). Endothelial Cell–Specific Inactivation of TSPAN12 (Tetraspanin 12) Reveals Pathological Consequences of Barrier Defects in an Otherwise Intact Vasculature. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(11), 2691-2705.

Publication 2018

mT/mG reporter mice

Alleles Animals Cdh5 Ert2 Genders Heterozygous Institutional animal care and use committee Knockout mice Mice Mice mice Transgene Vision

Corresponding Organization :

Other organizations : University of Colorado Boulder, University of Colorado Denver, University of Münster, Max Planck Institute for Molecular Biomedicine

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Variable analysis

independent variables

Cre-driver lines: Tg(Cdh5-cre/ERT2)1Rha, Pdgfrb(BAC)−CreERT2, and Rx-Cre

dependent variables

Conditional knockout mouse phenotypes

control variables

Animals of both genders were used for all studies
MT/mG reporter mice were obtained from Jackson Laboratories

controls

Positive control: mT/mG reporter mice
Negative control: Not explicitly mentioned

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