Preclinical CT Imaging with ExiTron Nano

The contrast agent used in the present study (Viscover™ ExiTron™ nano; Miltenyi Biotec, Bergisch-Gladbach, Germany) is an alkaline earth-based nanoparticulate contrast agent specifically formulated for pre-clinical computed tomography imaging. The nanoparticles are sterically stabilized by a polymer coating and have a mean hydrodynamic diameter of 110 nm. Upon i.v. injection, ExiTron nano circulates in the blood stream and is taken up by cells of the reticuloendothelial system (RES), including macrophages within the liver, the so-called Kupffer cells. Two different formulations of the contrast agent are available and both were used in the underlying study: ExiTron nano 6000 (optimized for liver/spleen imaging) and ExiTron nano 12000 (optimized for angiography) with densities of the undiluted contrast agents prior to injection of approx. 6000 HU and approx. 12000 HU, respectively. The injected volume of 100 µl ExiTron nano per mouse (25g) corresponds to a dose equivalent to 640 mg iodine/kg body weight or 1200 mg iodine/kg body weight for ExiTron nano 6000 and ExiTron nano 12000, respectively.

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Boll H., Nittka S., Doyon F., Neumaier M., Marx A., Kramer M., Groden C, & Brockmann M.A. (2011). Micro-CT Based Experimental Liver Imaging Using a Nanoparticulate Contrast Agent: A Longitudinal Study in Mice. PLoS ONE, 6(9), e25692.

Publication 2011

Angiography Blood stream Body weight Cells Computed tomography Contrast agents Hydrodynamic Iodine Kupffer cells Liver Mouse Polymer Reticuloendothelial system Spleen

Corresponding Organization :

Other organizations : Heidelberg University, University Hospital Heidelberg, University Medical Centre Mannheim, University of Giessen

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Variable analysis

independent variables

Contrast agent formulation (ExiTron nano 6000 vs. ExiTron nano 12000)

dependent variables

Uptake of contrast agent by cells of the reticuloendothelial system (RES), including Kupffer cells in the liver

control variables

Injected volume (100 µl ExiTron nano per mouse weighing 25g)
Density of undiluted contrast agents (approx. 6000 HU for ExiTron nano 6000 and approx. 12000 HU for ExiTron nano 12000)
Mouse weight (25g)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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