Standard immunohistochemistry and immunofluorescence protocols were used to examine protein expression. Primary antibodies used in this study included: Rabbit anti-Laminin α5 (1:800, generated as described previously [42 (link)]), Rat anti-Laminin α2 (1:400, Sigma, USA), Goat anti-Laminin α4 (1:400, R&D, USA), Rat anti-CD31 (1:100, BD Biosciences, USA), Mouse anti-Claudin-5 (1:200, Invitrogen, USA), Rabbit anti-ZO-1 (1:400, Thermofisher, USA), Rat anti-PDGFRβ (1:200, eBioscience, USA), Rabbit anti-AQP4 (1:500, Millipore, USA), IB4–488 (1:200, Thermofischer, USA), Rat anti-Ly6G (1:200; Biolegend, USA), Rat anti-CD68 (1:200, Biolegend, USA), Rabbit anti-glial fibrillary acidic protein (GFAP, 1:400, Stem cell, USA), and Rabbit anti-Iba1 (1:500; Wako Inc, USA). The fluorescence intensity of immunoreactivity and the number of immune-positive cells were calculated using the ImageJ software (NIH, USA). For quantifications, 3–5 random fields around the hematoma per section, 5 non-consecutive sections per animal, and at least 3 animals were used.