Chromatin Immunoprecipitation for Ikaros and H3K9me3

qChIP assays were performed by incubating chromatin with antibodies against Ikaros15 (link)16 (link)17 (link), H3K9me3 (Abcam, Cambridge, MA, USA) or normal rabbit IgG (Abcam) as a control15 (link)16 (link)17 (link)48 (link). Enrichment of the ChIP sample over input was evaluated by qPCR with ≥ 3 replicates using specific primers in the promoter region of DNM2(forward: 5′-ACCGCGGGATGGAAGAG-3′, reverse: 5′-TGAAGGCGTCCTGCAGTTT-3′). Relative concentration of the qPCR product is presented as the fold change of the level of DNA-Ikaros and DNA-H3K9me3 samples compared with controls.

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Ge Z., Gu Y., Han Q., Zhao G., Li M., Li J., Chen B., Sun T., Dovat S., Gale R.P, & Song C. (2016). Targeting High Dynamin-2 (DNM2) Expression by Restoring Ikaros Function in Acute Lymphoblastic Leukemia. Scientific Reports, 6, 38004.

Publication 2016

H3K9me3 normal rabbit IgG

Antibodies Assays Chip Chromatin Dnm2 Primers Rabbit

Corresponding Organization :

Other organizations : Zhongda Hospital Southeast University, Nanjing Medical University, Jiangsu Province Hospital, Temple University, Penn State Milton S. Hershey Medical Center, Pennsylvania State University, Imperial College London

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Variable analysis

independent variables

Antibodies against Ikaros
H3K9me3 antibody
Normal rabbit IgG

dependent variables

Enrichment of the ChIP sample over input
Relative concentration of the qPCR product

control variables

Chromatin
QPCR primers for the promoter region of DNM2

controls

Positive control: Antibodies against Ikaros
Negative control: Normal rabbit IgG

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