Immunohistochemical Characterization of Hepatic Cells

Optimal cutting temperature (OCT) compound embedded liver biopsy cryosections and paraffin-embedded sections (both 5 μm) were incubated with anti-NKp46 (Clone 195314, R&D Systems), anti-SMA-α (Rabbit polyclonal, Abcam) or TGF-β (Rabbit polyclonal, Abcam), respectively, according to our previously described protocols26 (link)38 (link). Double staining was performed using mouse anti-NKp46 and anti-SMA-α to evaluate the co-location of hepatic NK cells and HSCs. High-powered fields (hpf, 400×) were used to count positive cells. Positive cells were counted in three different fields by two independent observers.

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Shi J., Zhao J., Zhang X., Cheng Y., Hu J., Li Y., Zhao X., Shang Q., Sun Y., Tu B., Shi L., Gao B., Wang F.S, & Zhang Z. (2017). Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-β-dependent emperipolesis in HBV cirrhotic patients. Scientific Reports, 7, 44544.

Publication 2017

anti-NKp46 anti-SMA-α TGF-β

Biopsy Cells Clone Cryosections Hscs Liver Mouse Nk cells Nkp46 Paraffin Rabbit Tgf β

Corresponding Organization :

Other organizations : 302 Military Hospital of China, Peking University, 82th Hospital of Pla, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health

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Variable analysis

independent variables

Anti-NKp46 (Clone 195314, R&D Systems)
Anti-SMA-α (Rabbit polyclonal, Abcam)
TGF-β (Rabbit polyclonal, Abcam)

dependent variables

Number of positive cells

control variables

Cryosections and paraffin-embedded sections (both 5 μm)
High-powered fields (hpf, 400×)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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