Liver tissues obtained on day 3 postinfection were fixed in 10% buffered formalin, and 4 μM paraffin-embedded sections were stained with H&E. The specimen slides were scanned at ×20 magnification on an Aperio CS2 ScanScope (Leica Biosystems), and total, necrotic, and infiltration areas composed of lymphocytes and other mononuclear cells in the entire specimen were quantified using Aperio ImageScope software (Leica Biosystems) (16 (link), 26 (link)).
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Quantifying Liver Histopathology in Infection
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Corresponding Organization : Sidney Kimmel Cancer Center
Other organizations : University of Pennsylvania, University of California, Davis
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Variable analysis
independent variables
- Day 3 postinfection
- Total area
- Necrotic area
- Infiltration area composed of lymphocytes and other mononuclear cells
- Liver tissues
- 10% buffered formalin fixation
- Paraffin-embedded sections
- H&E staining
- ×20 magnification on Aperio CS2 ScanScope (Leica Biosystems)
- Aperio ImageScope software (Leica Biosystems) for quantification
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