Antibody Evaluation of EGP Extracts

The following antibodies were used for evaluation of the EGP extracts by western immunoblot^{15 (link),17 (link),29 (link)}: GHSR1a (n = 6 in each group), Sox9 (n = 8 in each group), PPARγ (n = 6 in each group) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), OX1R (n = 8 in each group) (Abcam, Cambridge, MA, USA), AKT (n = 6 in each group), p-AKT (n = 6 in each group), AMPK (n = 8 in each group), p-AMPK (n = 8 in each group) (Cell Signaling Technology, Danvers, MA, USA), and β-actin (MP Biomedical Solon, OH, USA). The full protocol is described in our previous publication^{29 (link)}. Serum TRAP 5b was measured using an ELISA kit (MBS704438; MyBioSource, Inc., San Diego, CA, USA). The low and high detection limits were 0.312 and 40 mlU/ml, and the coefficient of variation was <5%.

Free full text: Click here

Assadi M.H., Segev Y, & Tarasiuk A. (2020). Upper Airway Obstruction Elicited Energy Imbalance Leads to Growth Retardation that Persists after the Obstruction Removal. Scientific Reports, 10, 3206.

Publication 2020

GHSR1a PPARγ p-AKT p-AMPK β-actin

Actin Antibodies Elisa Ghsr1a Pparγ Serum Solon Sox9

Corresponding Organization : Ben-Gurion University of the Negev

Other organizations : Soroka Medical Center

Top 5 similar protocols

Variable analysis

independent variables

GHSR1a (n = 6 in each group)
Sox9 (n = 8 in each group)
PPARγ (n = 6 in each group)
OX1R (n = 8 in each group)
AKT (n = 6 in each group)
P-AKT (n = 6 in each group)
AMPK (n = 8 in each group)
P-AMPK (n = 8 in each group)

dependent variables

Serum TRAP 5b (measured using an ELISA kit)

control variables

β-actin (MP Biomedical Solon, OH, USA)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!