IF staining was performed based on published methods (39 (link), 40 (link)). Primary antibodies were used at 1:150 dilution as following: EYA2 (ab95875, Abcam), YBX1 (sc-101198, Santa Cruz), EGFR (sc-03, Santa Cruz) and PCNA (sc-7907, Santa Cruz). The goat anti-mouse and the goat anti-rabbit secondary antibodies (Alexa Fluor-568) were both used at 1:300. Cell nuclei were stained with Hoechst 33342 at the dilution of 1:1,000.
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