Giemsa Staining of EHEC-Infected HCMs

Giemsa staining of HCMs and bacteria was performed according to methods described previously by Rajan et al. (85 (link)) and Tatsuno et al. (86 (link)). HCMs were infected with EHEC strains as described above. Bacteria and infected cells were imaged under a Zeiss Primovert inverted microscope at a ×40 magnification. Bacterial clusters on HCMs containing >8 bacteria were considered MCs (86 (link)). The number of MCs was scored as the sum from 20 random microscopic fields. A technician counted the MCs in a blind manner to ensure objectivity.

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Yang D., Yang Y., Qiao P., Jiang F., Zhang X., Zhao Z., Cai T., Li G, & Cai W. (2023). Genomic Island-Encoded Histidine Kinase and Response Regulator Coordinate Mannose Utilization with Virulence in Enterohemorrhagic Escherichia coli. mBio, 14(2), e03152-22.

Publication 2023

Primovert inverted microscope

Bacteria Blind Cells Ehec Giemsa Microscopic Strains

Corresponding Organization : Iowa State University

Other organizations : Harbin Medical University, Second Affiliated Hospital of Harbin Medical University, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences

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