Immunohistochemical Analysis of Neurodegenerative Markers

A polyclonal rabbit anti-hepcidin 25 (Abcam ab30760) recognising a 2.8 kDa protein (Raha-Chowdhury et al., 2015 (link)), human anti-ferritin light chain (Abcam ab69090), human anti-ferritin heavy chain (Abcam ab65080), Anti-FPN (Abcam ab85370), anti-GFAP (Abcam ab48050), anti-CD68 (Sigma–Aldrich, MAB98073), and monoclonal anti-Iba1 (Thermo Fisher Scientific, MAB M1/70), Polyclonal anti-Iba1(Wako cat number 019-19741), monoclonal anti-IL-6 (Thermo Fisher, cat number M620), monoclonal anti-IL-1β (Thermo Fisher Scientific, cat number ILB1-H67), Anti-β amyloid (Covance cat number SIG 39320), Anti-Phospho-Tau (AT8, Thermo Fisher Scientific, cat number MN1020), was used for IHC. The following secondary antibodies were used: biotinylated goat anti-rabbit-Ig and biotinylated horse anti-mouse (both from Vector Laboratories, 1:250 for IHC); Alexa Fluor 568-labeled donkey anti-mouse-Ig, Alexa Fluor 488-labeled donkey anti-rabbit-Ig, and Alexa Fluor 568-labeled donkey anti-goat-Ig (all from Invitrogen, 1:1,000 for immunofluorescence).