FISH was carried out to study the mitotic chromosomes of root meristems. On the other hand, GISH was used to examine both the mitotic chromosomes of root meristemes and meiotic chromosomes of PMCs. Four probes were subjected to in situ hybridization on the same chromosome preparations. First FISH was made according to Książczyk et al. (2011 (link)) with minor modifications of Kwiatek et al. (2013 (link)), using 25S (used for detection of 25-5.8-18S rDNA loci) and 5S rDNA (pTa794). The hybridization mixture (40 μl per slide) contained 90 ng of each probe in the presence of salmon sperm DNA, 50 % formamide, 2 × SSC, 10 % dextran sulphate, and was denatured at 75 °C for 10 min and stored on ice for 10 min. Chromosomal DNA was denatured in the presence of the hybridization mixture at 75 °C for 5 min and allowed to hybridize overnight at 37 °C. For detection of the hybridization signals, anti-digoxigenin conjugated with FITC (Roche) was used. After documentation of the FISH sites, the slides were washed according to Heslop-Harrison (2000 (link)) (2 × 45 min in 4 × SSC Tween, 2 × 5 min in 2 × SSC, at room temperature).
Second FISH with pSc119.2 and pAs1 (labelled with digoxygenin-11-dUTP and tetramethyl-rhodamine-5-dUTP, respectively) was made with the same conditions after reprobing. After second reprobing, GISH was carried out according to Kwiatek et al. (2012 (link)) with modifications. Multicolour GISH was carried out using U-genome probe (from Ae. umbellulata), Sl-genome probe (from Ae. longissima) and unlabelled triticale genomic DNA which was used as specific blocker. The GISH mixture (40 μL per slide), containing 50 % formamide, 2 × SSC, 10 % dextran sulphate, 90 ng each of the genome probes, and 4.5 μg blocking DNA, was denatured at 75 °C for 10 min and stored on ice for 10 min. In case of initial GISH on triticale ‘Lamberto’ chromosomes, the hybridization mix contained the following: A-genome probe generated from genomic DNA of Triticum monococcum L., R-genome probe (rye, S. cereale L.) and blocking DNA from B-genome (Aegilops speltoides Tausch; 2n = 2x = 14; SS). The chromosomal DNA denaturation, hybridization and immunodetection conditions were the same as above-mentioned. Mitotic and meiotic (MI) cells were examined with an Olympus XM10 CCD camera attached to an Olympus BX 61 automatic epifluorescence microscope. Image processing was carried out using Olympus Cell-F (version 3.1; Olympus Soft Imaging Solutions GmbH: Münster, Germany) imaging software and PaintShop Pro X5 software (version 15.0.0.183; Corel Corporation, Ottawa, Canada). The identification of particular chromosomes were made by comparing the signal pattern of 5S rDNA, 25S rDNA, pSc119.2 and pAs1 probes according previous study (Kwiatek et al. 2013 (link)) and similar cytogenetic analysis (Cuadrado and Jouve 1994 (link); Schneider et al. 2003 (link), 2005 (link); Wiśniewska et al. 2013 (link)). Single-factor analysis of variance and Tukey’s Honest Significant Difference (HSD) test was used to examine the differences of means of chromosome configurations between plants from respective generations and the differences of means of chromosome configurations between plants from BC2F1 with comparison to their progeny in BC2F2 generation.
Second FISH with pSc119.2 and pAs1 (labelled with digoxygenin-11-dUTP and tetramethyl-rhodamine-5-dUTP, respectively) was made with the same conditions after reprobing. After second reprobing, GISH was carried out according to Kwiatek et al. (2012 (link)) with modifications. Multicolour GISH was carried out using U-genome probe (from Ae. umbellulata), Sl-genome probe (from Ae. longissima) and unlabelled triticale genomic DNA which was used as specific blocker. The GISH mixture (40 μL per slide), containing 50 % formamide, 2 × SSC, 10 % dextran sulphate, 90 ng each of the genome probes, and 4.5 μg blocking DNA, was denatured at 75 °C for 10 min and stored on ice for 10 min. In case of initial GISH on triticale ‘Lamberto’ chromosomes, the hybridization mix contained the following: A-genome probe generated from genomic DNA of Triticum monococcum L., R-genome probe (rye, S. cereale L.) and blocking DNA from B-genome (Aegilops speltoides Tausch; 2n = 2x = 14; SS). The chromosomal DNA denaturation, hybridization and immunodetection conditions were the same as above-mentioned. Mitotic and meiotic (MI) cells were examined with an Olympus XM10 CCD camera attached to an Olympus BX 61 automatic epifluorescence microscope. Image processing was carried out using Olympus Cell-F (version 3.1; Olympus Soft Imaging Solutions GmbH: Münster, Germany) imaging software and PaintShop Pro X5 software (version 15.0.0.183; Corel Corporation, Ottawa, Canada). The identification of particular chromosomes were made by comparing the signal pattern of 5S rDNA, 25S rDNA, pSc119.2 and pAs1 probes according previous study (Kwiatek et al. 2013 (link)) and similar cytogenetic analysis (Cuadrado and Jouve 1994 (link); Schneider et al. 2003 (link), 2005 (link); Wiśniewska et al. 2013 (link)). Single-factor analysis of variance and Tukey’s Honest Significant Difference (HSD) test was used to examine the differences of means of chromosome configurations between plants from respective generations and the differences of means of chromosome configurations between plants from BC2F1 with comparison to their progeny in BC2F2 generation.
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