Tungsten-Carbide Bead Milling and CTAB DNA Extraction

A 200 mg (dry weight) of each sample was transferred to a 2 ml microcentrifuge tube with two 3 mm tungsten-carbide beads (Qiagen) and ground to a fine powder on a Retsch mill MM 301 (Retsch GmbH, Haan Germany) (2X cycles for 45 s at 25 Hz). Total DNA was extracted from the homogenized contents using a modified CTAB extraction method as described by Doyle and Doyle (1990) , and adapted by Raclariu et al. (2017b) (link). The final elution volume was 100 μl.

Free full text: Click here

Raclariu-Manolică A.C., Anmarkrud J.A., Kierczak M., Rafati N., Thorbek B.L., Schrøder-Nielsen A, & de Boer H.J. (2021). DNA Metabarcoding for Quality Control of Basil, Oregano, and Paprika. Frontiers in Plant Science, 12, 665618.

Publication 2021

3 mm tungsten-carbide beads

Ctab Powder Tungsten carbide

Corresponding Organization : National Institute of Research and Development for Biological Sciences

Top 5 similar protocols

Variable analysis

independent variables

Amount of sample (200 mg dry weight)

dependent variables

Total DNA extracted

control variables

Size of microcentrifuge tube (2 ml)
Number of tungsten-carbide beads (2 x 3 mm)
Grinding method (Retsch mill MM 301, 2X cycles for 45 s at 25 Hz)
DNA extraction method (modified CTAB extraction method)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!