Chromatin immunoprecipitation assays were performed with SimpleChIP enzymatic ChIP kit (Cell Signaling) with anti-FoxO1 (SantaCruz) as described [72 (link)]. The immunoprecipitated DNA subjected to RT-PCR analysis with iTaq Universal SYBR Green Supermix (Bio-Rad) using the primer (SABiosciences, catalog no. GPM1030787(-)02A) that amplify a region of the mouse irf4 promoter containing the predicted FoxO1 binding sites. Data were analyzed with the LightCycler 480 software. The results were normalized to control IgG and input DNA.
AssaysBinding sites Chip Chromatin immunoprecipitation Enzymatic Irf4 Mouse Primer Rt pcr Sybr green
Corresponding Organization :
Other organizations :
The Ohio State University Wexner Medical Center, The Ohio State University, University of Illinois at Chicago, Pusan National University
Positive control: Chromatin immunoprecipitation with anti-FoxO1 antibody
Negative control: Chromatin immunoprecipitation with control IgG
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