Protocol detail

Culturing Human Embryonic Stem Cells

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Human ESCs (CSES37 (link)38 (link) and HUES14 (ref. 39 (link))) were cultured on mouse embryonic fibroblast treatment with mitomycin-C. Culture medium contained KnockOut Dulbecco's modified Eagle's medium (Gibco-Invitrogen, CA) supplemented with 15% KnockOut-SR (Gibco-Invitrogen, CA), 1 mM glutamine, 0.1 mM β-mercaptoethanol (Sigma-Aldrich, MO), 1% non-essential amino-acid stock (Gibco-Invitrogen, CA), penicillin (50 U ml⁻¹), streptomycin (50 μg ml⁻¹), and 8 ng ml⁻¹ fibroblast growth factor 2 (Gibco-Invitrogen, CA). Cells were passaged using trypsin-EDTA (Biological Industries, Beit Haemek, Israel).

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Weissbein U., Schachter M., Egli D, & Benvenisty N. (2016). Analysis of chromosomal aberrations and recombination by allelic bias in RNA-Seq. Nature Communications, 7, 12144.

Publication 2016

KnockOut Dulbecco's modified Eagle's medium KnockOut-SR glutamine β-mercaptoethanol non-essential amino-acid stock penicillin streptomycin fibroblast growth factor 2 trypsin-EDTA

Biological Cells Culture medium Edta Embryonic Escs Essential amino acid Fibroblast Fibroblast growth factor 2 Glutamine Human Mercaptoethanol Mitomycin c Mouse Penicillin Streptomycin Trypsin

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Variable analysis

independent variables

Culture medium composition
Cell line (CSES37, HUES14)

dependent variables

Not explicitly mentioned

control variables

Mouse embryonic fibroblast treatment with mitomycin-C
Passage method (trypsin-EDTA)

controls

Positive control: Not specified
Negative control: Not specified

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