In utero electroporation of mouse embryos

In utero electroporation was performed essentially as described previously [35 (link), 36 (link)]. Briefly, pregnant Institute of Cancer Research mice at E15.5 were anesthetized, and the uterine horns were exposed. Approximately 1 μl of DNA solution was injected into the lateral ventricles of embryos. The embryos were subjected to five square electric pulses (35 V, 50 ms, 1 Hz) using an electroporator (CUY21E; NEPA Gene). The brains with abnormal morphology were excluded from the experiments.

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Mori T., Kasem E.A., Suzuki-Kouyama E., Cao X., Li X., Kurihara T., Uemura T., Yanagawa T, & Tabuchi K. (2019). Deficiency of calcium/calmodulin-dependent serine protein kinase disrupts the excitatory-inhibitory balance of synapses by down-regulating GluN2B. Molecular Psychiatry, 24(7), 1079-1092.

Publication 2019

CUY21E

Brains Cancer Electric Electroporation Embryos Gene Lateral ventricles Mice Pulses Uterine horns Utero

Corresponding Organization :

Other organizations : Shinshu University, University of Tsukuba

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Variable analysis

independent variables

Electrical pulses (35 V, 50 ms, 1 Hz)

dependent variables

Morphology of the brains

control variables

Pregnant Institute of Cancer Research mice at E15.5
Injection of DNA solution into the lateral ventricles of embryos

controls

Brains with abnormal morphology were excluded from the experiments

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