The characterization of PHB was carried out through 1H & 13C NMR analysis38 (link)–40 (link). The 1H & 13 C NMR spectrum analysis was seized at 400 MHz using the Bruker Advance Model. The PHB sample was dissolved in 10 mg/ml−1 of deuteron chloroform (CDCl3) solvent used to interpret it.
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