Inflammatory Cytokine and Cigarette Smoke Exposure Protocol

Recombinant IL-1α, IL-36α, IL-36β, IL-36γ, IL-36Ra, TNF-α, neutrophil elastase, cathepsin G, proteinase-3, and anti–IL-36γ antibody (catalog AF2320) were purchased from R&D Systems. Rabbit anti–goat Ig/HRP (P0449) was purchased from Agilent. Poly(I:C) was purchased from Sigma-Aldrich. Nontypeable H. influenzae were obtained from the National Collection of Type Cultures (strain no. 1269) and were heat-killed by incubation at 65°C for 10 minutes as described previously (55 (link)). CSE was generated as previously described (56 (link)) from full-strength Marlboro cigarette (Phillip Morris). Budesonide was purchased from Thermo Fisher Scientific.

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Baker J.R., Fenwick P.S., Koss C.K., Owles H.B., Elkin S.L., Fine J., Thomas M., El Kasmi K.C., Barnes P.J, & Donnelly L.E. (2022). IL-36 receptor agonist and antagonist imbalance drives neutrophilic inflammation in COPD. JCI Insight, 7(15), e155581.

Publication 2022

Recombinant IL-1α IL-36α IL-36β IL-36γ IL-36Ra TNF-α neutrophil elastase proteinase-3 Poly(I:C) Budesonide

Anti antibody Budesonide Cathepsin g Goat H influenzae Neutrophil elastase Poly i c Proteinase Rabbit Strain Tnf α

Corresponding Organization : Imperial College London

Other organizations : Boehringer Ingelheim (Germany), Imperial College Healthcare NHS Trust, Boehringer Ingelheim (United States)

Top 5 similar protocols

Variable analysis

independent variables

Recombinant IL-1α
IL-36α
IL-36β
IL-36γ
IL-36Ra
TNF-α
Neutrophil elastase
Cathepsin G
Proteinase-3
Anti–IL-36γ antibody (catalog AF2320)
Poly(I:C)
Nontypeable H. influenzae
Cigarette smoke extract (CSE)
Budesonide

dependent variables

Not explicitly mentioned

control variables

Rabbit anti–goat Ig/HRP (P0449)

positive controls

Nontypeable H. influenzae

negative controls

Not explicitly mentioned

Annotations

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