HEK293T (ATCC CRL-3216), U2OS (ATCC HTB-96), U2OS-265 (34 (link)) and U2OS DR-GFP (ATCC CRL-3455) cells were grown in DMEM (ThermoFisher Scientific 10569044) supplemented with 10% FBS (R&D solutions S11150) at 37°C with 5% CO2. U2OS-265 DSB reporter cells were a generous gift from Roger Greenberg (34 (link)).
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Culturing Various Cell Lines
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Corresponding Organization : Winthrop Rockefeller Foundation
Other organizations : Missouri University of Science and Technology, The University of Texas Health Science Center at San Antonio
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Variable analysis
independent variables
- Cell lines used: HEK293T (ATCC CRL-3216), U2OS (ATCC HTB-96), U2OS-265 (34 (link)), and U2OS DR-GFP (ATCC CRL-3455)
- Not explicitly mentioned
- Growth media: DMEM (ThermoFisher Scientific 10569044) supplemented with 10% FBS (R&D solutions S11150)
- Incubation conditions: 37°C with 5% CO2
- Positive control: None mentioned
- Negative control: None mentioned
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