Immunofluorescence staining was performed as described previously [38 (link)] using anti-SSEA1 (Santa Cruz; sc-21,702; 1:250), anti-Nestin (Santa Cruz; sc-33,677; 1:250), anti-GFAP (Sigma; G9269; 1:1,000), or anti-POU3F4 (BRN4) antibody (Proteintech; 25114-1-AP; 1:500). RNA extraction, cDNA synthesis, and qPCR were performed as described above except using random primers for cDNA synthesis. Primers are listed in Table S2.
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