Pancreatic Cancer Cell Culture Protocols

MiaPaCa-2 was purchased from ATCC. The murine cell line Panc02 was from Dr. Min Li (26 (link)). The murine cell line KPC was from Dr. Vonderheide (27 (link)). Cell line authentication was performed using short tandem repeat profiling. The cell lines tested negative for mycoplasma contamination. Cells were cultured in 5% CO₂ at 37°C in Dulbecco’s modified Eagle’s medium (DMEM, Cellgro, Herndon, VA) containing 10% fetal bovine serum (Gemini, Woodland, CA) and 50 U/ml penicillin/streptomycin (Cellgro, Herndon, VA).

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Bakst R.L., Xiong H., Chen C.H., Deborde S., Lyubchik A., Zhou Y., He S., McNamara W., Lee S.Y., Olson O.C., Leiner I.M., Marcadis A.R., Keith J.W., Al-Ahmadie H.A., Katabi N., Gil Z., Vakiani E., Joyce J.A., Pamer E, & Wong R.J. (2017). Inflammatory monocytes promote perineural invasion via CCL2-mediated recruitment and cathepsin B expression. Cancer research, 77(22), 6400-6414.

Publication 2017

MiaPaCa-2 Dulbecco’s modified Eagle’s medium (DMEM, penicillin/streptomycin

Cell line authentication Cell lines Cells Eagle Fetal bovine serum Murine Mycoplasma Penicillin Short tandem repeat Streptomycin Woodland

Corresponding Organization : Memorial Sloan Kettering Cancer Center

Other organizations : Icahn School of Medicine at Mount Sinai, Technion – Israel Institute of Technology, Ludwig Cancer Research, University of Lausanne

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Variable analysis

independent variables

Cell line

dependent variables

Cell growth/proliferation
Mycoplasma contamination

control variables

Culture conditions (5% CO2, 37°C)
Culture media (DMEM with 10% FBS and 50 U/ml penicillin/streptomycin)

controls

Positive control: None mentioned
Negative control: Cells tested negative for mycoplasma contamination

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