Furosemide, lipase, minimum essential medium, oleic acid, Tween 80®, Tween 20®, polyethylene glycol, and glycerol were purchased from Sigma Aldrich (St. Louis, MO, USA). Gattefossé, Neuilly sur-Seine, France, gifted Cremophor EL®, Transcutol HP®, and Cremophor RH 40®. All other reagents used were of analytical grade.
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