Western Blotting Analysis of Protein Targets

Western blotting (WB) was performed according to previous reports [13 (link)]. The primary antibodies used were anti-β-actin (#4970, Cell Signaling Technology, Danvers, MA, USA), anti-NRAS (#10724-1-AP, Proteintech, Chicago, IL, USA), anti-DOHH (#254866, Abcam, Cambridge, MA, USA), anti-HIST1H2AC (#15953-1-AP, proteintech), anti-TAF6 (#ab76922, Abcam), anti-p44/42 MAPK (Erk1/2) (#4695, Cell Signaling Technology), and anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#9101, Cell Signaling Technology). Each primary antibody was diluted to 1:1000 and incubated overnight. The secondary antibody, diluted to 1:5000, was incubated at room temperature for 1 h. Protein signals were detected using ChemiDoc XRS Plus Systems (Bio-Rad, Hercules, CA, USA) after a 5 min reaction with the ECL substrate (Bio-Rad). Original images of Western blot analysis please see Supplementary File S1.