Saliva specimens were collected for NICU-HEALTH participants in the morning and afternoon on a single day each week during the NICU stay. Collections were conducted by trained study staff. The methods of sample collection, storage, and cortisol measurement have been described previously18 (link). In brief, saliva specimens were collected using a cotton swab (SalivaBio Infant Swab, Salimetrics) placed in the infant’s mouth for 2–5 min until the cotton swab was saturated. The swab was then centrifuged at 4,000 rotations per minute for 30 min, and extracted saliva was aliquotted to storage vials and frozen at −80 °C pending batch analysis. Saliva specimens were sent frozen to the Technical University of Dresden for cortisol measurement using a commercially available chemiluminescence assay38 –39 (link).
Protocol detail
Saliva Cortisol Measurement in NICU Infants
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Corresponding Organization : Icahn School of Medicine at Mount Sinai
Other organizations : Cohen Children's Medical Center, Northwell Health
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Variable analysis
independent variables
- None explicitly mentioned
- Saliva cortisol levels
- Time of day (morning and afternoon) for saliva collection
- Sample collection, storage, and measurement methods
- Use of trained study staff for sample collection
- Positive control: Not mentioned
- Negative control: Not mentioned
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