Measuring Soluble ICAM-1 Levels

We previously evaluated sICAM (soluble intercellular adhesion molecule) [18 (link)]. Measurement was drawn from the same routine blood sample in S-Monovette 9 mL, Clotting Activator/Serum (Sarstedt, Nümbrecht, Germany, ref. 02.1063) and was allowed to clot for 30 min. After centrifugation at 2500× g for 10 min at room temperature, the supernatant was collected in aliquots and frozen at −80 °C until analysis. Soluble ICAM-1 levels were measured using the human ICAM-1/CD54 nonallele-specific Quantikine ELISA kit (Cat. no. SCIM00, R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions. Every blood sample was measured in duplicate.