ChIP-Seq Analysis of H3K27ac in NB4 Cells
Corresponding Organization :
Other organizations : Soochow University
Variable analysis
- Formaldehyde concentration (1%)
- Formaldehyde crosslinking duration (10 min)
- Glycine concentration (1.25 M)
- Glycine neutralization duration (5 min)
- Anti-histone H3 (acetyl K27) antibody
- Chromatin fragmentation by sonication
- Chromatin immunoprecipitation (ChIP) with anti-histone H3 (acetyl K27) antibody
- DNA purification and sequencing
- Cell line (NB4)
- Room temperature
- Overnight incubation at 4 °C
- Alignment to reference genome (UCSC hg38)
- Peak calling using MACS2 software
- Visualization using Integrative Genomics Viewer (IGV)
- Positive control: Not specified
- Negative control: Not specified
Annotations
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