Ascl1-Driven Neuronal Fate Mapping

Ascl1 infected Tau-EGFP reporter MEFs were FAC-sorted 5, 7, 10, 12 or 22 days post-Ascl1 induction with dox. RNA was then extracted from both Tau-EGFP positive and negative populations from each time point, as well as uninfected control MEFs and unsorted d2 Ascll infected MEFs using the TRIzol RNA isolation protocol (Invitrogen, 15596-018). Reverse transcription into cDNA was performed using the SuperScript III First-strand Synthesis System (Invitrogen, 18080-051) and qRT-PCR was performed using Sybr Green (Thermo Fisher Scientific, 4309155). Immunostaining was performed as previously described^{8 (link)}. Antibodies and qRT-PCR primers are listed in the Supplementary Information.