ERG was performed on mice following 4 h of dark adaption. Pupils were dilated with 0.5% tropicamide and 1.25% phenylephrine then mice were anesthetized with a combined mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg). Scotopic ERGs were recorded using an Espion E2 system (Diagnosys), at increasing flash intensities, as described previously (83 (link)). Flash responses were smoothed using a low-pass noise-filtering script before analysis. The amplitude heights of the A-wave and B-wave for each ERG flash response were calculated and fitted using a previously published equation (83 (link)) in OriginPro 9.0 (OriginLab), as previously described (31 (link), 42 (link), 44 (link), 47 (link)). Bmax1 and Bmax2 values were calculated as described (31 (link)).
Protocol detail
Dark-Adapted Scotopic ERG Analysis
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Other organizations : Duke University
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Variable analysis
independent variables
- Flash intensity
- A-wave amplitude
- B-wave amplitude
- Dark adaptation duration (4 h)
- Pupil dilation with 0.5% tropicamide and 1.25% phenylephrine
- Anesthesia with ketamine (100 mg/kg) and xylazine (10 mg/kg)
- Scotopic ERG recording using Espion E2 system
- Smoothing of flash responses using low-pass noise-filtering script
- Analysis using previously published equation in OriginPro 9.0
- No positive or negative controls were explicitly mentioned.
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