Fluorescent IHC Analysis of Tumor Xenografts

Expression of Ki67 in tumor xenografts was determined by fluorescent immunohistochemistry on frozen sections to examine the effect of G-1 on the proliferation of breast cancer cells in vivo (46 (link)). Fluorescent immunohistochemistry was also used to determine tubulin distribution and phosphorylated histone H3. Images were captured with a Zeiss 710 Meta Confocal Laser Scanning Microscope and analyzed with the Zeiss Zen 2010 software.

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Lv X., He C., Huang C., Hua G., Wang Z., Remmenga S.W., Rodabough K.J., Karpf A.R., Dong J., Davis J.S, & Wang C. (2017). G-1 inhibits breast cancer cell growth via targeting colchicine-binding site of tubulin to interfere with microtubule assembly. Molecular cancer therapeutics, 16(6), 1080-1091.

Publication 2017

710 Meta Confocal Laser Scanning Microscope

Cancer of breast Cells proliferation Confocal laser scanning microscope Frozen sections Histone h3 Immunohistochemistry Tubulin Tumor Xenografts

Corresponding Organization : University of Nebraska Medical Center

Other organizations : Huazhong Agricultural University, First Affiliated Hospital of Zhengzhou University

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Protocol cited in 3 other protocols

Variable analysis

dependent variables

Expression of Ki67 in tumor xenografts
Tubulin distribution
Phosphorylated histone H3

control variables

Fluorescent immunohistochemistry on frozen sections

controls

No positive or negative controls were explicitly mentioned.

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