Antibody Detection of Seizure Proteins

N-terminal antibody against seizure protein 6 (Sez6) was described previously [10 (link), 21 (link)]. For detection of seizure 6-like protein (Sez6L) we used monoclonal antibody 21D9 [19 (link)]. Additionally, immunoblotting of mouse brain tissue lysates was performed using the following antibodies: anti-BACE1 (D10E5; Cell Signaling); anti-APP (22C11; Merck Millipore) for flAPP and sAPPt; anti-sAPPβ (SIG39138; Covance) and anti-actin (Sigma Aldrich). For immunohistochemistry of mouse brain tissue, the following antibodies were used: anti-BACE1 (Epitomics; Abcam), anti-APP (Y188; Epitomics, Abcam), anti-GFAP (Dako), anti-CD45 (BD Biosciences) and anti-calbindin (Swant). Immunocytochemistry of primary mouse cortical neurons was performed using these antibodies: anti-BACE1 (Epitomics; Abcam), anti-EEA1 (Cell Signaling), anti-transferrin receptor (TfR; ThermoFisher Scientific) and anti-LAMP1 (1D4B, Santa Cruz Biotechnology).

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Causevic M., Dominko K., Malnar M., Vidatic L., Cermak S., Pigoni M., Kuhn P.H., Colombo A., Havas D., Flunkert S., McDonald J., Gunnersen J.M., Hutter-Paier B., Tahirovic S., Windisch M., Krainc D., Lichtenthaler S.F, & Hecimovic S. (2018). BACE1-cleavage of Sez6 and Sez6L is elevated in Niemann-Pick type C disease mouse brains. PLoS ONE, 13(7), e0200344.

Publication 2018

anti-BACE1 (D10E5; anti-APP (22C11; anti-actin anti-GFAP anti-CD45 anti-calbindin anti-BACE1 anti-EEA1 anti-transferrin receptor (TfR;

Actin Antibodies Antibody Bace1 Brain Calbindin Cortical Gfap Immunocytochemistry Immunohistochemistry Lamp1 Monoclonal antibody Mouse Neurons Protein Protein n Seizure Tissue Transferrin receptor

Corresponding Organization : Munich Cluster for Systems Neurology

Top 5 similar protocols

Variable analysis

independent variables

N-terminal antibody against seizure protein 6 (Sez6)
Monoclonal antibody 21D9 for detection of seizure 6-like protein (Sez6L)
Anti-BACE1 (D10E5; Cell Signaling)
Anti-APP (22C11; Merck Millipore) for flAPP and sAPPt
Anti-sAPPβ (SIG39138; Covance)
Anti-actin (Sigma Aldrich)
Anti-BACE1 (Epitomics; Abcam)
Anti-APP (Y188; Epitomics, Abcam)
Anti-GFAP (Dako)
Anti-CD45 (BD Biosciences)
Anti-calbindin (Swant)
Anti-EEA1 (Cell Signaling)
Anti-transferrin receptor (TfR; ThermoFisher Scientific)
Anti-LAMP1 (1D4B, Santa Cruz Biotechnology)

dependent variables

Detection of seizure 6-like protein (Sez6L)
Immunoblotting of mouse brain tissue lysates
Immunohistochemistry of mouse brain tissue
Immunocytochemistry of primary mouse cortical neurons

control variables

Mouse brain tissue lysates
Mouse brain tissue
Primary mouse cortical neurons

controls

Positive control: None specified
Negative control: None specified

Annotations

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