The lysates of Ishikawa and HEC1-A cells were reacted with the magnetic bead (Millipore, Bedford, MA, USA) and anti-AGO2 (1:500, ab186733, Abcam) or control anti-IgG. Then immunoprecipitated RNA was purified, and the enrichment of circSMAD2 and miR-1277-5p were evaluated by qRT-PCR.
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Investigating circSMAD2 and miR-1277-5p Interactions
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Other organizations : Shanxi Medical University
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Variable analysis
independent variables
- Cell type (Ishikawa and HEC1-A cells)
- Enrichment of circSMAD2 and miR-1277-5p
- Anti-AGO2 (1:500, ab186733, Abcam)
- Control anti-IgG
- Positive control: Anti-AGO2 immunoprecipitation
- Negative control: Control anti-IgG immunoprecipitation
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