In both villages, trapped mosquitoes were morphologically identified using the keys published by Gillies and Coetzee (1987 ), counted, and the data entered in MS Excel spreadsheets. Culicine mosquitoes were identified up to genus level, and anophelines into An. gambiae sensu lato, An. funestus and other anopheline species. Abdominal statuses of female mosquitoes were categorized as unfed, blood-fed, or gravid. Mosquitoes belonging to the An. gambiae complex were identified to species using a ribosomal DNA Polymerase Chain Reaction assay (Scott et al., 1993 ).
Mosquito Ecology in Kenyan Villages
In both villages, trapped mosquitoes were morphologically identified using the keys published by Gillies and Coetzee (1987 ), counted, and the data entered in MS Excel spreadsheets. Culicine mosquitoes were identified up to genus level, and anophelines into An. gambiae sensu lato, An. funestus and other anopheline species. Abdominal statuses of female mosquitoes were categorized as unfed, blood-fed, or gravid. Mosquitoes belonging to the An. gambiae complex were identified to species using a ribosomal DNA Polymerase Chain Reaction assay (Scott et al., 1993 ).
Corresponding Organization :
Other organizations : International Centre of Insect Physiology and Ecology, Wageningen University & Research
Protocol cited in 6 other protocols
Variable analysis
- Location (Lwanda village and Kigoche village)
- Mosquito abundance and species composition
- Mosquito abdominal status (unfed, blood-fed, or gravid)
- Altitude (1,169 m for Lwanda, 1,160 m for Kigoche)
- Average rainfall (1,200 mm for Lwanda, 1,000-1,800 mm for Kigoche)
- Relative humidity (65% for both villages)
- Mean temperature (18-34°C for Lwanda, 17-32°C for Kigoche)
- Mosquito breeding sites (hoof prints, rice irrigation scheme)
- Occupations (fishing, livestock keeping in Lwanda; rice cultivation in Kigoche)
- Housing characteristics (mud-walled with open eaves, corrugated iron-sheet roofs, no ceiling, single- or double-roomed)
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