The laminin overlay assay was performed as previously described (35 (link)). Briefly, PVDF membranes were blocked in laminin-binding buffer (LBB: 10 mM triethanolamine, 140 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.6) containing 5% non-fat dry milk, followed by incubation with 7.5 nM mouse Engelbreth–Holm–Swarm (EHS) laminin (Sigma) at 4°C overnight in LBB containing 3% BSA. Subsequently, membranes were washed and incubated with rabbit anti-laminin antibody (Sigma) at 4°C for 3 h, followed by anti-rabbit IgG–HRP at room temperature for 1 h.
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