5×103 UACC 903 and 1205 Lu melanoma cells were seeded in 96-well plates, followed by treatment with 0.62 to 100 μmol/L of empty control or abietic acid nanoliposomes or Nanolipolee-007 for 24 hours. Percentage proliferating or apoptotic cells were quantified by a colorimetric cell proliferation ELISA BrdU kit (Roche Applied Sciences, Indianapolis, IN) or fluorimetric Apo-ONE Homogenous caspase-3/7 assay kit as previously reported (Promega, Madison, WI) (21 (link)).
Protocol detail
Evaluating Abietic Acid Nanoliposomes for Melanoma
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Abietic acid
Apo 3
Apoptotic
Assay
Brdu
Caspase
Caspase 7
Cell proliferation
Cells
Colorimetric
Elisa
Fluorimetric
Homogenous
Melanoma
Promega
Corresponding Organization : Pennsylvania State University
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Variable analysis
independent variables
- Empty control nanoliposomes
- Abietic acid nanoliposomes
- Nanolipolee-007
- Concentrations of nanoliposomes (0.62 to 100 μmol/L)
- Percentage of proliferating cells
- Percentage of apoptotic cells
- UACC 903 and 1205 Lu melanoma cells
- 96-well plates
- Treatment duration of 24 hours
- Positive control: Not specified
- Negative control: Empty control nanoliposomes
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