DIANA-microT server has been updated to miRBase v18 and Ensembl v69. The server is compatible with the new miRNA nomenclature (3p/5p) introduced in miRBase v18, as well as with previous miRNA naming conventions. It currently supports 7.3 × 106H.sapiens, 3.5 × 106 M.musculus, 4.4 × 105D.melanogaster and 2.5 × 105 C.elegans interactions between 3876 miRNAs and 64 750 protein-coding genes. Gene (9 (link)) and miRNA (13 (link)) expression annotation has been incorporated into the web server, enabling the user to perform advanced result filtering based on tissue expression. Furthermore, users can also restrict predictions between uploaded lists of expressed genes and/or miRNAs. For example, this feature can be used to identify interactions between a list of repressed (or overexpressed) genes and overexpressed (or repressed) miRNAs, in the case of a differential expression analysis pipeline.
Moreover, the web server hosts an updated version of the KEGG database providing a relevant search module based on KEGG pathway descriptions (14 (link)). A redesigned optional user space has also been implemented, which provides personalized features and facilitates the interconnectivity between the web server and the available DIANA software and databases (Figure 1).