Tissue Microarray Analysis of CRC

Tissue microarray (TMA) blocks covering 21 randomly selected CRC and 6 controls from adjacent morphologically normal tissue were cut into 4-mm sections and arrayed according to the Microarray technique with a semi-automatic ATA-100 Chemicon system (Chemicon’s Advanced Tissue Array).^{18 (link)} TMAs were processed for IHC as previously described,^{11 (link)} using as primary antibodies anti-YY1 (1/500), anti-Fas (1/250) or normal IgG serum (negative control) (all from Santa Cruz Biotechnology, Santa Cruz, CA). Immunostaining was digitized and analyzed by an Aperio Scanscope CS (Aperio, Vista, CA), as previously described.^{11 (link)} The sample collection and use was approved by the Ethics Committee of the Children Hospital of Mexico ‘Federico Gomez’ (Mexico City, Mexico). Both CRC and normal samples were collected by the Department of Pathology of Hospital General Regional No. 25, IMSS and Speciality Hospital CMN ‘La Raza’, IMSS (Mexico City, Mexico). All CRC cases were classified according to the Dukes or TNM staging.

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Pothoulakis C., Torre-Rojas M., Duran-Padilla M.A., Gevorkian J., Zoras O., Chrysos E., Chalkiadakis G, & Baritaki S. (2017). CRHR2/Ucn2 signaling is a novel regulator of miR-7/YY1/Fas circuitry contributing to reversal of colorectal cancer cell resistance to Fas-mediated apoptosis. International journal of cancer, 142(2), 334-346.

Publication 2017

anti-YY1 anti-Fas

Anti antibodies Children Hospital ethics committee Microarray Sample collection Serum Tissue

Corresponding Organization : University of Crete

Other organizations : Hospital Infantil de México Federico Gómez, Hospital General de México

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Variable analysis

independent variables

Tissue microarray (TMA) blocks covering 21 randomly selected CRC and 6 controls from adjacent morphologically normal tissue

dependent variables

YY1 expression
Fas expression

control variables

4-mm sections of TMA blocks
IHC processing as previously described
Immunostaining digitized and analyzed by an Aperio Scanscope CS

controls

Negative control: normal IgG serum

Annotations

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