Invasion Assay Using Matrigel Chambers

Four hours after transfection, normal (10% FBS) cell medium was replaced with 2% FBS media. The following day, Corning BioCoat growth factor reduced Matrigel invasion chambers (Corning; New York, USA) were warmed and rehydrated. Initial experiments evaluated optimum cell density for each line (data not shown), and invasion assays were carried out as we have described previously (34 (link)), before Eosin Y solution (Sigma-Aldrich) staining and being photographed on an EVOS XL Core Imaging System (ThermoFisher Scientific).

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Nieto H.R., Thornton C.E., Brookes K., Nobre de Menezes A., Fletcher A., Alshahrani M., Kocbiyik M., Sharma N., Boelaert K., Cazier J.B., Mehanna H., Smith V.E., Read M.L, & McCabe C.J. (2021). Recurrence of Papillary Thyroid Cancer: A Systematic Appraisal of Risk Factors. The Journal of Clinical Endocrinology and Metabolism, 107(5), 1392-1406.

Publication 2021

BioCoat growth factor reduced Matrigel invasion chambers Eosin Y solution EVOS XL Core Imaging System

Assays Cell Eosin y Growth factor Matrigel Transfection

Corresponding Organization : University of Birmingham

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Variable analysis

independent variables

Cell density for each cell line (initial experiments evaluated optimum cell density)

dependent variables

Cell invasion through Corning BioCoat growth factor reduced Matrigel invasion chambers

control variables

Cell culture medium (4 hours after transfection, normal medium replaced with 2% FBS medium)
Matrigel invasion chambers (Corning BioCoat growth factor reduced Matrigel invasion chambers were warmed and rehydrated)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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